Candidate gene markers associated with fecal secretion of feline enteric coronavirus (FECV)

Complete original article on portal of the US National Center for Biotechnology Information; 17.11.2020


Feline coronavirus (FCoV) can cause a fatal disease - feline infectious peritonitis. Cats that permanently shed this virus are the most important sources of infection. The role of the host in faecal excretion of FCoV is unknown. The aim of this study was to identify gene markers and test their association with FCoV secretion patterns. Fecal samples were taken from 57 cats of 12 breeds at baseline and then at 2, 4 and 12 months. Variations from persistent and / or high intensity excretion to no excretion were observed. Thirteen immune-related genes were selected as functional and positional / functional candidates. Positional candidates were selected in the candidate area detected by GWAS analysis. Tens to hundreds of single nucleotide polymorphisms (SNPs) per gene have been identified by next generation sequencing. Associations with different phenotypes were evaluated by chi-square tests and Fisher's exact tests. SNP of one functional and one position candidate (NCR1 and SLX4IP ) and haplotypes of four genes ( SNX5 , NCR2 , SLX4IP , NCR1 ) were associated with FCoV excretion at cor<0.01. Highly significant associations have been observed in extreme phenotypes (persistent / high-intensity secretors and non-excretors), suggesting that there are two major phenotypes associated with different genotypes - highly susceptible cats that persistently excrete high levels of viral particles, and coronavirus-resistant cats. do not exclude.

Keywords: feline enteric coronavirus, faecal excretion patterns, polymerase chain reaction, genetic susceptibility, candidate immune-related genes, association study